5D7U
Crystal structure of the C-terminal domain of MMTV integrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 35.988, 42.279, 139.087 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.772 - 1.500 |
| R-factor | 0.1613 |
| Rwork | 0.159 |
| R-free | 0.20710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ex4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2091: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.360 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.043 | 0.585 |
| Number of reflections | 17494 | |
| <I/σ(I)> | 29.2 | 3.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 12.2 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20% isopropanol, 0.2M Ammonium Acetate, 0.1M HEPES, pH7.5 |
