5D7H

X-RAY CRYSTAL STRUCTURE OF L,D TRANSPEPTIDASE 2 FROM MYCOBACTERIUM TUBERCULOSIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	61.265, 95.579, 75.550
Unit cell angles	90.00, 92.59, 90.00

Refinement procedure

Resolution	48.630 - 2.490
R-factor	0.165
Rwork	0.162
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.072
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.630
High resolution limit [Å]	2.490
Number of reflections	29821
<I/σ(I)>	22.14
Completeness [%]	97.5
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	16% PEG 5000 MME 0.18 M Ammonium Sulfate