5D7E
Crystal structure of Taf14 YEATS domain in complex with H3K9ac
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-06 |
Detector | NOIR-1 |
Wavelength(s) | 0.98 |
Spacegroup name | P 65 |
Unit cell lengths | 113.164, 113.164, 26.524 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.000 - 1.900 |
R-factor | 0.1863 |
Rwork | 0.185 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.220 |
Data reduction software | d*TREK |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.520 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.048 | 0.660 |
Number of reflections | 15763 | |
<I/σ(I)> | 30.8 | 3 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 10.2 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.2 M sodium citrate and 48% PEG600 (v/v) |