5D78

Structure of RRM3 Domain of Mip6 at 1.25 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97947
Spacegroup name	P 1 21 1
Unit cell lengths	25.842, 47.023, 30.563
Unit cell angles	90.00, 109.79, 90.00

Refinement procedure

Resolution	28.758 - 1.251
R-factor	0.1391
Rwork	0.137
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d77
RMSD bond length	0.009
RMSD bond angle	1.254
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.020	1.270
High resolution limit [Å]	1.250	1.250
Rmerge	0.310
Number of reflections	14132
<I/σ(I)>	24.6
Completeness [%]	74.1
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	294	0.2 M Lithium Sulfate; 0.1 M tris pH 8.5; 40% PEG 400