5D6G
CRYSTAL STRUCTURE OF FRAGMENT OF RIBOSOMAL PROTEIN P0 IN COMPLEX WITH 74NT 23S RNA FROM METHANOCOCCUS JANNASCHII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.918410 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 104.230, 108.310, 112.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.827 - 3.300 |
| R-factor | 0.2475 |
| Rwork | 0.245 |
| R-free | 0.29850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jsy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.435 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.143 | 0.790 |
| Number of reflections | 10828 | |
| <I/σ(I)> | 7.15 | 1.94 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 4.56 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 50 mM Sodium Cacodylate, pH 6.5, 0.2 M KCl, 0.1 M Magnesium acetate, 9% PEG 6000, 0.5 mM CTAB |
