5D6B
Human fumarase (wild type)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.459 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 124.420, 147.150, 57.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.670 - 2.100 |
| R-factor | 0.1827 |
| Rwork | 0.180 |
| R-free | 0.23930 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.670 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.179 |
| Number of reflections | 27725 |
| <I/σ(I)> | 3.6 |
| Completeness [%] | 89.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 100 mM sodium malonate, 12% polyethylene glycol (PEG) 3350 |
