5D6A
2.7 Angstrom Crystal Structure of ABC transporter ATPase from Vibrio vulnificus in Complex with Adenylyl-imidodiphosphate (AMP-PNP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 104.957, 104.957, 85.354 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.550 - 2.700 |
R-factor | 0.19991 |
Rwork | 0.194 |
R-free | 0.25555 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.051 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.117 | 0.621 |
Number of reflections | 15243 | |
<I/σ(I)> | 32.5 | 5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | Protein: 5.9 mg/ml, 0.5M Sodium chloride, 0.01M Tris-HCL (pH 8.3), 1mM AMPPNP; Screen: PACT (C2), 0.1M PCB buffer (pH 5.0), 25% (w/v) PEG 1500 |