5D67
Crystal structure of an EF-Hand calcium binding domain of CAP-Binding Protein Complex-Interacting Protein 1 (EFCAB6) from Homo sapiens at 2.00 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.407, 67.187, 70.697 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 27.118 - 2.000 |
| R-factor | 0.1704 |
| Rwork | 0.169 |
| R-free | 0.20330 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.920 |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.118 | 2.070 | |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.112 | 0.036 | 0.869 |
| Rmeas | 0.130 | 0.041 | 1.064 |
| Total number of observations | 197737 | ||
| Number of reflections | 26723 | 5348 | 4446 |
| <I/σ(I)> | 10.59 | 32.2 | 1.3 |
| Completeness [%] | 98.1 | 99.4 | 84.3 |
| Redundancy | 3.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30% polyethylene glycol 4000, 0.2M ammonium sulfate |
