5D60
Structure of Chaetomium thermophilum Skn7 coiled-coil domain, crystal form III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.730, 40.202, 52.777 |
Unit cell angles | 90.00, 118.57, 90.00 |
Refinement procedure
Resolution | 44.550 - 1.900 |
R-factor | 0.2432 |
Rwork | 0.240 |
R-free | 0.29470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d5y |
RMSD bond length | 0.019 |
RMSD bond angle | 1.757 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.27) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.550 | 44.550 | 1.940 |
High resolution limit [Å] | 1.900 | 9.090 | 1.900 |
Rmerge | 0.069 | 0.050 | 0.599 |
Rpim | 0.048 | 0.035 | 0.407 |
Total number of observations | 41428 | 365 | 2597 |
Number of reflections | 14684 | ||
<I/σ(I)> | 7.2 | 16.3 | 1.7 |
Completeness [%] | 97.8 | 92.5 | 96.6 |
Redundancy | 2.8 | 2.5 | 2.8 |
CC(1/2) | 0.998 | 0.999 | 0.722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 25 % PEG-3350, 0.2 M MgCl2 and 0.1 M Bis-Tris pH 5.5 |