5D5W

Crystal structure of Chaetomium thermophilum Skn7 with HSE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97702
Spacegroup name	C 2 2 21
Unit cell lengths	39.771, 78.397, 90.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.350
R-factor	0.2173
Rwork	0.215
R-free	0.25010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hts
RMSD bond length	0.006
RMSD bond angle	1.044
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.042	45.042	2.480
High resolution limit [Å]	2.349	7.430	2.349
Rmerge		0.019	0.958
Rmeas	0.051
Rpim	0.023	0.010	0.469
Total number of observations	28671	885	4202
Number of reflections	6090
<I/σ(I)>	17.4	52.3	1.8
Completeness [%]	98.9	96.3	99.2
Redundancy	4.7	4	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	11 % PEG-3350, 0.18 M tri-Ammonium Citrate