5D5R
Horse-heart myoglobin - deoxy state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 280 |
| Detector technology | PIXEL |
| Collection date | 2015-05-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.600, 28.800, 35.600 |
| Unit cell angles | 90.00, 106.50, 90.00 |
Refinement procedure
| Resolution | 34.213 - 1.600 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cmv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.074 | 0.478 |
| Number of reflections | 16486 | |
| <I/σ(I)> | 14.3 | 2.9 |
| Completeness [%] | 96.8 | 87.9 |
| Redundancy | 5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 3.5 M ammonium sulphate, 0.1 M Tris-HCl pH 7.4 |
