5D5G
Structure of colocasia esculenta agglutinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2014-12-05 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5419 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 122.012, 47.198, 82.251 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.986 - 1.740 |
R-factor | 0.3455 |
Rwork | 0.344 |
R-free | 0.42720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r0e |
RMSD bond length | 0.008 |
RMSD bond angle | 1.376 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.000 | 1.580 |
High resolution limit [Å] | 1.536 | 1.536 |
Rmerge | 0.380 | 0.610 |
Number of reflections | 59836 | |
<I/σ(I)> | 2.7 | 0.9 |
Completeness [%] | 83.4 | 20 |
Redundancy | 5.61 | 1.92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | reservoir included 1.8 M ammonium sulphate and 0.1 M Hepes, pH 6.0; stock protein solution contained 8.1 mg/ml of lectin in 20 mM Tris pH 8.5; protein & reservoir soln mixed in 3:1 ratio at start and placed on siliconized cover slip |