5D5C
In meso in situ serial X-ray crystallography structure of lysozyme at 100 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.090, 79.090, 38.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.545 - 1.700 |
R-factor | 0.1854 |
Rwork | 0.183 |
R-free | 0.22190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tmu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.022 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 13594 | |
<I/σ(I)> | 6.16 | 1.1 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.5 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.5-1 M NaBr, 50-100 mM CH3COONa, pH 4.5, and 15-30 %(v/v) PEG400 |