5D55
Crystal structure of the E. coli Hda pilus minor tip subunit, HdaB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.6531 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 112.294, 112.294, 61.652 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.400 - 2.000 |
R-factor | 0.20735 |
Rwork | 0.203 |
R-free | 0.24831 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4or1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.184 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0062) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.400 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.371 |
Number of reflections | 26601 | |
<I/σ(I)> | 9.3 | 2.7 |
Completeness [%] | 98.1 | 95.1 |
Redundancy | 3.6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 0.2M Ammonium Citrate, 20% PEG 3350 |