5D54
In meso X-ray crystallography structure of insulin at 100 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.03321 |
Spacegroup name | I 21 3 |
Unit cell lengths | 77.510, 77.510, 77.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.755 - 1.500 |
R-factor | 0.1781 |
Rwork | 0.177 |
R-free | 0.18830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 9ins |
RMSD bond length | 0.006 |
RMSD bond angle | 0.920 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.540 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 12580 | |
<I/σ(I)> | 15.45 | 1.49 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.46 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.1-0.2 M sodium phosphate, pH 5.5-6.1, and 33-38 %(w/v) PEG400 |