5D4Z
Crystal structure of Repressor from Salmonella-temperate phage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.599, 62.497, 267.896 |
| Unit cell angles | 89.99, 89.97, 72.70 |
Refinement procedure
| Resolution | 49.130 - 2.980 |
| R-factor | 0.21855 |
| Rwork | 0.216 |
| R-free | 0.27324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d50 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.254 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.980 |
| Number of reflections | 75973 |
| <I/σ(I)> | 22.8 |
| Completeness [%] | 98.6 |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 296 | 15%(w/v) PEG 4000, 0.1M magnesium sulfate |
