5D4Y
A psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.386, 78.442, 88.724 |
Unit cell angles | 90.00, 100.49, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.500 |
R-factor | 0.19584 |
Rwork | 0.191 |
R-free | 0.28480 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.580 |
Data reduction software | Coot |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 24211 |
<I/σ(I)> | 43.1 |
Completeness [%] | 97.6 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 298 | 0.1M Tris, pH 8.5, 0.2M Li2SO4, 30% (w/v) PEG 4000 |