5D4L
Structure of the apo form of CPII from Thiomonas intermedia K12, a nitrogen regulatory PII-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-13 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 3 |
| Unit cell lengths | 82.690, 82.690, 31.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.806 - 2.300 |
| R-factor | 0.1801 |
| Rwork | 0.177 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cz4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 71.610 | 2.360 | |
| High resolution limit [Å] | 2.300 | 10.270 | 2.300 |
| Rmerge | 0.128 | 0.038 | 0.698 |
| Rmeas | 0.134 | 0.040 | 0.733 |
| Total number of observations | 121103 | ||
| Number of reflections | 10491 | 118 | 747 |
| <I/σ(I)> | 16.96 | 55.5 | 3.24 |
| Completeness [%] | 97.4 | 99.2 | 91.9 |
| Redundancy | 11.5 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 20% PEG 3500, 0.15 M LD Malic Acid pH 7.0 |
