5D4L

Structure of the apo form of CPII from Thiomonas intermedia K12, a nitrogen regulatory PII-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-10-13
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 3
Unit cell lengths	82.690, 82.690, 31.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.806 - 2.300
R-factor	0.1801
Rwork	0.177
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cz4
RMSD bond length	0.008
RMSD bond angle	1.228
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.610		2.360
High resolution limit [Å]	2.300	10.270	2.300
Rmerge	0.128	0.038	0.698
Rmeas	0.134	0.040	0.733
Total number of observations	121103
Number of reflections	10491	118	747
<I/σ(I)>	16.96	55.5	3.24
Completeness [%]	97.4	99.2	91.9
Redundancy	11.5		10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	20% PEG 3500, 0.15 M LD Malic Acid pH 7.0