5D43
crystal structrue of Mouse centrin 1 in calcium-saturated form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-31 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.750, 72.061, 62.248 |
| Unit cell angles | 90.00, 97.26, 90.00 |
Refinement procedure
| Resolution | 46.890 - 2.820 |
| R-factor | 0.27683 |
| Rwork | 0.274 |
| R-free | 0.32943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ggm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.136 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.750 | 2.970 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Number of reflections | 10469 | |
| <I/σ(I)> | 11.5 | 4 |
| Completeness [%] | 99.4 | 98.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | PEG 3350, Hepes, Calcium acetate, Ethylene glycol |
