5D3A
KIF21A regulatory coiled coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 2.066410 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.020, 82.020, 70.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.030 - 2.495 |
| R-factor | 0.1785 |
| Rwork | 0.175 |
| R-free | 0.20230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.465 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.030 | 2.584 |
| High resolution limit [Å] | 2.495 | 2.495 |
| Rmerge | 0.176 | 1.785 |
| Number of reflections | 9871 | |
| <I/σ(I)> | 15.84 | |
| Completeness [%] | 100.0 | |
| Redundancy | 17.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293.15 | 0.1 M citric acid, 10 % w/v PEG 6000 |
