5D2U
Crystal structure of tPphA Variant - H39A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 38.042, 153.872, 82.761 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.230 - 1.800 |
R-factor | 0.2206 |
Rwork | 0.217 |
R-free | 0.25970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xzv |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.230 |
High resolution limit [Å] | 1.800 |
Number of reflections | 105414 |
<I/σ(I)> | 19 |
Completeness [%] | 99.8 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 298 | 100mM Tris-HCl, pH 7.4, 200mM CaCl2, 26%(v/v) PEG3350 |