5D2Q

Crystal structure of STPR from Bombyx Mori in complex with 20-bp DNA derived from +290 site of the fibroin gene

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	45.890, 45.890, 240.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.080 - 2.400
R-factor	0.24915
Rwork	0.247
R-free	0.29595
RMSD bond length	0.016
RMSD bond angle	1.792
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	10911
<I/σ(I)>	12.3
Completeness [%]	99.8
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	22% PEG 3350, 16% Glycerol,0.2 M ammonium citrate pH7.0