5D2Q
Crystal structure of STPR from Bombyx Mori in complex with 20-bp DNA derived from +290 site of the fibroin gene
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 45.890, 45.890, 240.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.080 - 2.400 |
R-factor | 0.24915 |
Rwork | 0.247 |
R-free | 0.29595 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.792 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 10911 |
<I/σ(I)> | 12.3 |
Completeness [%] | 99.8 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 22% PEG 3350, 16% Glycerol,0.2 M ammonium citrate pH7.0 |