5D2C
Reaction of phosphorylated CheY with imidazole 1 of 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-13 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.620, 53.720, 162.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.200 - 2.060 |
| R-factor | 0.1622 |
| Rwork | 0.161 |
| R-free | 0.18500 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 81.200 |
| High resolution limit [Å] | 2.060 |
| Number of reflections | 29630 |
| <I/σ(I)> | 15.1 |
| Completeness [%] | 97.6 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | TRIS, MnCl2, BeCl2, NaF,(NH4)2SO4, imidazole, glycerol |
