5D1Z

IsdB NEAT1 bound by clone D4-10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	90
Detector technology	CCD
Collection date	2014-07-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 2 21 21
Unit cell lengths	115.292, 147.005, 165.045
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.960 - 3.170
R-factor	0.23605
Rwork	0.233
R-free	0.28712
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.857
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.960
High resolution limit [Å]	3.170
Number of reflections	45212
<I/σ(I)>	7.6
Completeness [%]	97.9
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		290	17% PEG 3350 0.2M Ammonium Citrate Tri