5D1Q

IsdB NEAT2 bound by clone D2-06

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	90
Detector technology	CCD
Collection date	2014-07-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 42 21 2
Unit cell lengths	121.387, 121.387, 193.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.330 - 3.220
R-factor	0.24118
Rwork	0.239
R-free	0.28838
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.471
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.330
High resolution limit [Å]	3.220
Number of reflections	22680
<I/σ(I)>	20.4
Completeness [%]	98.8
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	20% PEG3350 0.1M Na Citrate pH 5.4