5D1K
Crystal Structure of UbcH5B in Complex with the RING-U5BR Fragment of AO7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.470, 45.658, 67.954 |
| Unit cell angles | 90.00, 118.09, 90.00 |
Refinement procedure
| Resolution | 33.339 - 1.780 |
| R-factor | 0.1707 |
| Rwork | 0.170 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2esk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.086 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.840 |
| High resolution limit [Å] | 1.780 | 3.830 | 1.780 |
| Rmerge | 0.057 | 0.028 | 0.467 |
| Number of reflections | 27367 | ||
| <I/σ(I)> | 11.8 | 69.124 | 3.013 |
| Completeness [%] | 99.1 | 99.9 | 92.7 |
| Redundancy | 7 | 7.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 20% PEG-10000, 8% ethylene glycol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
