5D1I
Structure of Cyclic nucleotide-binding-like protein from Brucella abortus bv. 1 str. 9-941
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9762 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 56.096, 82.911, 103.918 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.682 - 2.000 |
R-factor | 0.1877 |
Rwork | 0.185 |
R-free | 0.23320 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.728 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX (dev_1819) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.085 | 0.054 | 0.381 |
Rmeas | 0.087 | 0.055 | 0.388 |
Rpim | 0.016 | 0.011 | 0.072 |
Total number of observations | 478013 | ||
Number of reflections | 16773 | ||
<I/σ(I)> | 11.9 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 20 | 26.7 | 15 |
CC(1/2) | 0.998 | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | Potassium sodium tartrate tetrahydrate,olyethylene glycol 3350, KCl |