5D1H
Crystal structure of the 16S rRNA (adenine(1408)-N(1))-methyltransferase W203A mutant from Catenulisporales acidiphilia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.007, 84.112, 94.368 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.056 - 2.803 |
R-factor | 0.188 |
Rwork | 0.183 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x1o |
RMSD bond length | 0.003 |
RMSD bond angle | 0.754 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.8.4_1496) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.099 | |
Number of reflections | 13446 | |
<I/σ(I)> | 15.6 | 2.6 |
Completeness [%] | 92.5 | 92.4 |
Redundancy | 4.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2 M Ammonium Acetate, 0.1 M Sodium Acetate Trihydrate, 30% PEG |