5D17
Structure of the C-terminal domain of TnsE at 2.85 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.330, 87.125, 141.621 |
| Unit cell angles | 90.00, 95.01, 90.00 |
Refinement procedure
| Resolution | 39.749 - 2.850 |
| R-factor | 0.2214 |
| Rwork | 0.221 |
| R-free | 0.25050 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.756 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.114 | 1.000 |
| Number of reflections | 122729 | |
| <I/σ(I)> | 21 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG 3350, NaCl, TRIS |
