5D0Y
Substrate bound S-component of folate ECF transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.92017 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.910, 77.540, 89.450 |
| Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
| Resolution | 43.012 - 3.014 |
| R-factor | 0.2376 |
| Rwork | 0.235 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d3m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.429 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.155 | 1.160 |
| Number of reflections | 21443 | |
| <I/σ(I)> | 4.3 | 2.18 |
| Completeness [%] | 82.4 | 0.75 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 24% 350 PEG MME, 100mM Tris |
