5D0Q

BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	197
Detector technology	PIXEL
Collection date	2015-05-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 42 21 2
Unit cell lengths	254.157, 254.157, 179.215
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.943 - 3.500
R-factor	0.3154
Rwork	0.314
R-free	0.33990
RMSD bond length	0.006
RMSD bond angle	1.186
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	SHELXDE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.940	3.580
High resolution limit [Å]	3.500	3.500
Number of reflections	74050
<I/σ(I)>	8.2
Completeness [%]	99.8	100
Redundancy	26.9	24.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	297	0.1 M Tris, pH8.0, 0.1 M MgCl2, 24% PEG500 MME.