5D0O
BamABCDE complex, outer membrane beta barrel assembly machinery entire complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 196 |
Detector technology | PIXEL |
Collection date | 2015-03-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.8233 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 116.689, 116.689, 435.192 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.653 - 2.900 |
R-factor | 0.2776 |
Rwork | 0.276 |
R-free | 0.30410 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.809 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.650 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.180 | |
Number of reflections | 67553 | |
<I/σ(I)> | 15 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.4 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, pH7.5, 30% PEG600, CYMAL-4 |