5D0F
Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (E564Q) in complex with maltopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.070, 202.010, 135.240 |
| Unit cell angles | 90.00, 101.32, 90.00 |
Refinement procedure
| Resolution | 49.053 - 3.300 |
| R-factor | 0.1941 |
| Rwork | 0.192 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d06 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.021 |
| Data reduction software | MOSFLM (7.2.0) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | MOLREP (11.2.08) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 132.609 | 49.053 | 3.480 |
| High resolution limit [Å] | 3.300 | 10.440 | 3.300 |
| Rmerge | 0.029 | 0.488 | |
| Rmeas | 0.108 | 0.036 | 0.596 |
| Rpim | 0.059 | 0.020 | 0.335 |
| Total number of observations | 187312 | 6441 | 24625 |
| Number of reflections | 59857 | ||
| <I/σ(I)> | 8.5 | 23.1 | 2.1 |
| Completeness [%] | 96.1 | 93.3 | 94.8 |
| Redundancy | 3.1 | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 10% PEG 5000 MME, 0.1 M Hepes, 5% tacsimate pH 7.0 |
