5D06
Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0391 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 159.271, 198.950, 261.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.753 - 3.100 |
| R-factor | 0.2349 |
| Rwork | 0.234 |
| R-free | 0.26820 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.737 |
| Data reduction software | MOSFLM (7.2.0) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHENIX (1.9-1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 130.775 | 48.217 | 3.270 |
| High resolution limit [Å] | 3.100 | 9.800 | 3.100 |
| Rmerge | 0.064 | 0.482 | |
| Rmeas | 0.128 | 0.076 | 0.574 |
| Rpim | 0.067 | 0.041 | 0.305 |
| Total number of observations | 242017 | 7224 | 35915 |
| Number of reflections | 73294 | ||
| <I/σ(I)> | 7.4 | 14.2 | 2 |
| Completeness [%] | 97.8 | 90.3 | 99.3 |
| Redundancy | 3.3 | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 10% PEG 5000 MME, 0.1 M Hepes pH 7.0, 5% tacsimate pH 7.0 |
