5CZL

Crystal structure of a novel GH8 endo-beta-1,4-glucanase from an Achatina fulica gut metagenomic library

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-03
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458
Spacegroup name	P 21 21 21
Unit cell lengths	50.424, 76.374, 95.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.600 - 2.391
R-factor	0.2034
Rwork	0.201
R-free	0.24750
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.600		2.540
High resolution limit [Å]	2.390	7.100	2.390
Rmerge	0.159	0.082
Rmeas	0.176	0.092	1.335
Total number of observations	79003
Number of reflections	14898	651	2203
<I/σ(I)>	7.02	16.44	1.38
Completeness [%]	97.9	97.5	91.8
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% PEG1000 10% PEG8000