5CZH
EGFR L858R MUTANT IN COMPLEX WITH AN OPTIMAL PEPTIDE SUBSTRATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97925 |
Spacegroup name | I 2 3 |
Unit cell lengths | 144.566, 144.566, 144.566 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.080 - 2.800 |
R-factor | 0.193 |
Rwork | 0.192 |
R-free | 0.22000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2itv |
RMSD bond length | 0.010 |
RMSD bond angle | 1.146 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.080 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.062 | 0.473 |
Number of reflections | 12368 | |
<I/σ(I)> | 16.6 | 2.2 |
Completeness [%] | 98.8 | 99.3 |
Redundancy | 3.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.1M HEPES 7.6, 0.15M NACL, 40% PEG400, 5MM TCEP, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |