5CYR
Crystal structure of Thosea asigna virus RNA-dependent RNA polymerase (RdRP) complexed with ATP and ssRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2012-05-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 142.513, 158.339, 217.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.599 - 3.504 |
R-factor | 0.2539 |
Rwork | 0.252 |
R-free | 0.29560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xhi |
RMSD bond length | 0.004 |
RMSD bond angle | 0.894 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 3.629 |
High resolution limit [Å] | 3.504 | 3.504 |
Rmerge | 0.310 | |
Number of reflections | 27974 | |
<I/σ(I)> | 5.57 | 2.3 |
Completeness [%] | 89.6 | 75.92 |
Redundancy | 8.1 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG 8000, Lithium sulfate |