5CYO

High resolution Septin 9 GTPase domain in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 1 21 1
Unit cell lengths	57.497, 78.049, 77.443
Unit cell angles	90.00, 105.92, 90.00

Refinement procedure

Resolution	55.292 - 2.035
R-factor	0.1856
Rwork	0.183
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.857
Data reduction software	MOSFLM
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.000		2.160
High resolution limit [Å]	2.035	6.070	2.035
Rmerge	0.075	0.027	0.724
Rmeas	0.091	0.033	0.875
Total number of observations	131988
Number of reflections	41927	1606	6631
<I/σ(I)>	11.2	32.11	1.99
Completeness [%]	99.0	97.2	97.4
Redundancy	3.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG1500, 20% Glycerol