5CYG

Crystal Structure of isoform 2 of uridine phosphorylase from Schistosoma mansoni APO form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-11-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	57.697, 76.277, 118.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.828 - 2.017
R-factor	0.1982
Rwork	0.196
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cyf
RMSD bond length	0.003
RMSD bond angle	0.708
Data reduction software	XDS
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.140
High resolution limit [Å]	2.017	5.810	2.017
Rmerge	0.078	0.046	0.304
Rmeas	0.092	0.054	0.369
Total number of observations	114824
Number of reflections	32883	1539	4546
<I/σ(I)>	10.07	22.29	2.65
Completeness [%]	93.0	94.9	81.4
Redundancy	3.49

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	25% PEG2000 MME