5CYB
Structure of a lipocalin lipoprotein affecting virulence in Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 107.485, 107.485, 63.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.100 - 2.100 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ge2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.100 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | |
| Number of reflections | 13101 | |
| <I/σ(I)> | 7.88 | 2.52 |
| Completeness [%] | 99.1 | 89.2 |
| Redundancy | 6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291.15 | 0.1M Na Acetate pH 4.6 0.01 Zn Chloride 18% PEG 6000 |
