5CY8
Structure of Xoo1075, a peptide deformylase from Xanthomonas oryzae pv oryze, in complex with fragment 244
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.99 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 58.661, 58.661, 263.749 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.650 - 2.380 |
R-factor | 0.18851 |
Rwork | 0.187 |
R-free | 0.22693 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e5d |
RMSD bond length | 0.019 |
RMSD bond angle | 2.148 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.420 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.430 | |
Number of reflections | 11737 | |
<I/σ(I)> | 25 | 4.5 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 9.7 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 0.05M Cadmium sulfate, 0.1M HEPES, 2.0M Sodium acetate trihydrate, pH 7.5 |