5CY5

Crystal structure of the T33-51H designed self-assembling protein tetrahedron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 2 3
Unit cell lengths	106.700, 106.700, 106.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.448 - 3.400
R-factor	0.1616
Rwork	0.156
R-free	0.19710
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1nog 1wy1
RMSD bond length	0.008
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.448		3.480
High resolution limit [Å]	3.400	15.190	3.400
Rmerge	0.092	0.038	1.359
Rmeas	0.094	0.039	1.388
Total number of observations	154895
Number of reflections	5827	81	428
<I/σ(I)>	29.04	78.16	2.79
Completeness [%]	99.9	98.8	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.8	298	100 mM HEPES pH 8.8 and 15 % PEG 3350