5CY2
Tn3 resolvase - site III complex crystal form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2006-03-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97948 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.900, 151.920, 106.140 |
Unit cell angles | 90.00, 99.85, 90.00 |
Refinement procedure
Resolution | 49.247 - 4.000 |
R-factor | 0.2094 |
Rwork | 0.207 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1000212338 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (dev_2067) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 4.070 |
High resolution limit [Å] | 4.000 | 4.000 |
Number of reflections | 19099 | |
<I/σ(I)> | 22.9 | 1 |
Completeness [%] | 99.5 | 96 |
Redundancy | 3.5 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | complex + well mixed 1:1 complex: 3mg/ml in 25mM tris pH 7.5 and 0.14M (NH4)2SO4 well: 16% PEG3350, 0.2M ammonium flouride cryoprotectant: added 20% ethylene glycol |