5CXT
Crystal structure of a RNA-binding protein 39 (RBM39) in complex with fragment of splicing factor (U2AF) from Unknown at 2.20 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 127.283, 127.283, 78.864 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.432 - 2.200 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s6e |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.635 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.432 | 39.432 | 2.260 |
| High resolution limit [Å] | 2.200 | 9.830 | 2.200 |
| Rmerge | 0.116 | 0.036 | 0.830 |
| Rmeas | 0.135 | 0.041 | 0.981 |
| Total number of observations | 275854 | ||
| Number of reflections | 72455 | 793 | 5269 |
| <I/σ(I)> | 9.71 | 26.6 | 2 |
| Completeness [%] | 99.7 | 98.3 | 98 |
| Redundancy | 3.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M potassium chloride, 15.0% polyethylene glycol monomethyl ether 5000, 0.1M HEPES pH 7.0 |
