5CXC
Structure of Ytm1 bound to the C-terminal domain of Erb1 in P 65 2 2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 169.110, 169.110, 154.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.818 - 3.100 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4U7A and 3PSL |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.820 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.280 | 2.817 |
| Number of reflections | 24180 | |
| <I/σ(I)> | 17 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 39.1 | 40.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 294 | 15% PEG 4000,0.1M Na citrate pH 5.6, 0.2M Ammonium sulfate |
