5CX3
Crystal structure of FYCO1 LIR in complex with LC3A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.426, 115.257, 73.517 |
Unit cell angles | 90.00, 102.29, 90.00 |
Refinement procedure
Resolution | 34.615 - 2.300 |
R-factor | 0.1811 |
Rwork | 0.178 |
R-free | 0.24450 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.161 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.7.0029) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 25680 |
<I/σ(I)> | 16.9 |
Completeness [%] | 99.1 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 289 | 1.2 M sodium citrate tribasic dihydrate, 0.1 M HEPES sodium |