5CX2
Structure of coiled coil domain of Leishmania donovani coronin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.390, 48.780, 45.350 |
| Unit cell angles | 90.00, 110.95, 90.00 |
Refinement procedure
| Resolution | 42.350 - 2.210 |
| R-factor | 0.23603 |
| Rwork | 0.233 |
| R-free | 0.28885 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.369 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 4.240 | 1.970 |
| Rmerge | 0.118 | 0.075 | |
| Total number of observations | 90732 | ||
| Number of reflections | 14275 | ||
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 87.3 | 100 | 40.8 |
| Redundancy | 6.4 | 7.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 279 | 3 ul of 8 mg/ml purified protein + 3 ul of reservoir solution (0.85M Ammonium Sulphate, 0.8M Lithium Sulphate, 10mM Sodium Citrate) equilibrated against 1ml reservoir. Crystals appeared in 7 - 10 days. |
