5CWU
Crystal structure of Chaetomium thermophilum Nup188 TAIL domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-11-02 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.97944 |
Spacegroup name | P 1 |
Unit cell lengths | 65.276, 65.278, 155.548 |
Unit cell angles | 90.00, 89.95, 90.01 |
Refinement procedure
Resolution | 19.853 - 3.350 |
R-factor | 0.2477 |
Rwork | 0.246 |
R-free | 0.27920 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.734 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 3.350 |
Number of reflections | 33102 |
<I/σ(I)> | 23.4 |
Completeness [%] | 99.0 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 294 | 100 mM HEPES, pH 6.9 16 % (w/v) PEG 3,350 200 mM ammonium citrate |