5CWT
Crystal structure of Chaetomium thermophilum Nup57
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.252, 42.361, 138.709 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.256 - 2.500 |
R-factor | 0.2461 |
Rwork | 0.241 |
R-free | 0.29260 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.002 |
RMSD bond angle | 0.443 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 8970 |
<I/σ(I)> | 58.3 |
Completeness [%] | 98.0 |
Redundancy | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 5 % (w/v) PEG 1,000 12 % (w/v) PEG 8,000 |