5CW9
Crystal structure of De novo designed ferredoxin-ferredoxin domain insertion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2014-07-31 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 80.563, 80.563, 222.683 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.282 - 3.108 |
R-factor | 0.3349 |
Rwork | 0.333 |
R-free | 0.34880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.711 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.282 | 3.420 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.077 | |
Number of reflections | 6783 | |
<I/σ(I)> | 18.4 | 5.1 |
Completeness [%] | 99.5 | 100 |
Redundancy | 13.1 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.1 M HEPES pH 7.5, 3.5 M NaCl |