5CW9

Crystal structure of De novo designed ferredoxin-ferredoxin domain insertion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2014-07-31
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	I 41 2 2
Unit cell lengths	80.563, 80.563, 222.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.282 - 3.108
R-factor	0.3349
Rwork	0.333
R-free	0.34880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.711
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.282	3.420
High resolution limit [Å]	3.100	3.100
Rmerge	0.077
Number of reflections	6783
<I/σ(I)>	18.4	5.1
Completeness [%]	99.5	100
Redundancy	13.1	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1 M HEPES pH 7.5, 3.5 M NaCl